Co-Factor Binding Confers Substrate Specificity to Xylose Reductase from Debaryomyces hansenii
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چکیده
منابع مشابه
Co-Factor Binding Confers Substrate Specificity to Xylose Reductase from Debaryomyces hansenii
Binding of substrates into the active site, often through complementarity of shapes and charges, is central to the specificity of an enzyme. In many cases, substrate binding induces conformational changes in the active site, promoting specific interactions between them. In contrast, non-substrates either fail to bind or do not induce the requisite conformational changes upon binding and thus no...
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Short-chain dehydrogenase reductases (SDRs) have been utilized for catalyzing the reduction of many aromatic/aliphatic prochiral ketones to their respective alcohols. However, there is a paucity of data that elucidates their innate biological role and diverse substrate space. In this study, we executed an in-depth biochemical characterization and substrate space mapping (with 278 prochiral keto...
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Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Edward M. Devine, Jr. Lipids of Debaryomyces hansenii. J. Bacteriol. 89:1488-1493. 1965.-The separation of neutral lipids and phospholipids from the lipid extract of Debaryomyces hansenii NRRL Y-1448 was accomplished by using a single column packed with silicic acid. The neutral lipids comprised 67%, and the phospholipids 33%, of the...
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The production of xylitol from concentrated synthetic xylose solutions (S(o) = 130-135 g/L) by Debaryomyces hansenii was investigated at different pH and temperature values. At optimum starting pH (pH(o) = 5.5), T = 24 degrees C, and relatively low starting biomass levels (0.5-0.6 g(x)/L), 88% of xylose was utilized for xylitol production, the rest being preferentially fermented to ethanol (10%...
متن کاملPhysiological and enzymatic study of Debaryomyces hansenii growth on xylose and oxygen limited chemostats
The effect of the growth rate and oxygen transfer rate on Debaryomyces hansenii physiology was studied using xylose-limited and oxygen-limited chemostat cultures respectively, and complemented with enzymatic assays. Under xylose-limited chemostat (oxygen-excess) neither ethanol nor xylitol was produced over the entire range of dilution rate (D). The maximal volumetric biomass productivity was 2...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0045525